Equipment and Materials

Chapter

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Chapter 6
Last Updated
2026-06-01
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2 minute(s)
Word Count
225 words

Table of Contents

Ancestors

Siblings

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Equipment and Materials

A.

  1. Same as for Salmonella, Chapter 5
  2. Forced air incubators, maintained at 42.0 ± 0.3°C and 44.0 ± 0.3°C
  3. Anaerobic jar
  4. Anaerobic gas generating pouch/sachet
  5. Anaerobic indicator

Media

B.

  1. Shigella broth with novobiocin (M136)
  2. Trypticase soy-yeast extract (TSYE) broth (M157)
  3. MacConkey agar (M91)
  4. Triple sugar iron (TSI) agar (M149)
  5. Urea broth (M171)
  6. Motility test medium (semisolid) (M103)
  7. Potassium cyanide (KCN) broth (M126)
  8. Malonate broth (M92)
  9. Tryptone (tryptophane) broth, 1% (M164)
  10. MR-VP broth (M104)
  11. Christensen citrate agar (M39)
  12. Veal infusion agar (M173)
  13. Bromcresol purple broth (M26) supplemented with the following carbohydrates, each at a level of 0.5%: adonitol, salicin, rhamnose, glucose, inositol, lactose, mannitol, raffinose, sucrose, xylose, dulcitol, and glycerol.
  14. Acetate agar (M3)
  15. Mucate broth (M105)
  16. Mucate control broth (M106)
  17. Decarboxylase basal medium (lysine, Falkow) (M44)
  18. Decarboxylase basal medium (ornithine)(M44)

Reagents and Stains

C.

  1. Kovacs' reagent (R38)
  2. Voges-Proskauer test reagents (R89)
  3. 1 N Sodium hydroxide solution (R73)
  4. 1 N Hydrochloric acid (R36)
  5. Methyl red indicator (R44)
  6. Physiological saline solution, 0.85% (sterile) (R63)
  7. Novobiocin
  8. Polyvalent Shigella antisera for groups A, A1, B, C, C1, C2, D and Alkalescens-Dispar biotypes 1-4
  9. Gram stain reagents(R32)