Chapter 1
- Mechanical Blender
- Several types are available. Use blender that has several operating speeds or rheostat.
- The term "high-speed blender" designates mixer with 4 canted, sharp-edge, stainless steel blades rotating at bottom of 4 lobe jar at 10,000-12,000 rpm or with equivalent shearing action.
- Suspended solids are reduced to fine pulp by action of blades and by lobular container, which swirls suspended solids into blades.
- Waring blender, or equivalent, meets these requirements.
- Sterile glass or metal high-speed blender jar
- 1000 ml, with cover, resistant to autoclaving for 60 min at 121°C
- Balance, with weights
- Sensitivity to 0.1%
- Sterile Beakers
- 250 ml, low-form, covered with aluminum foil
- Sterile Graduated Pipets
- 1.0 and 10.0 ml
- Butterfield's phosphate-buffered dilution water (Rll)
- Sterilized in bottles to yield final volume of 90 ± 1 ml
- Sterile knives, forks, spatulas, forceps, scissors, tablespoons, and tongue depressors
- For sample handling.