Same basic equipment as for conventional plate count (Chapter 3).
Drying cabinet or incubator for drying surface of agar plates 3.
Sterile bent glass streaking rods, hockey stick or hoe-shaped, with fire-polished ends, 3-4 mm diameter, 15-20 cm long, with an angled spreading surface 45-55 mm long
Media and Reagents
B.
Baird-Parker medium (M17)
Trypticase (tryptic) soy agar (TSA) (M152)
Brain heart infusion (BHI) broth (M24)
Coagulase plasma (rabbit) with EDTA
Toluidine blue-DNA agar (M148)
Lysostaphin (Schwartz-Mann, Mountain View Ave., Orangeburg, NY 10962)
Tryptone yeast extract agar (M165)
Paraffin oil, sterile
0.02 M phosphate-saline buffer (R61), containing 1% NaCl