- Oxygen and mammalian cell culture: are we repeating the experiment of Dr. Ox?
Ast et al. 2019
Commentary
- "Oxygen tensions vary widely across tissues but are significantly lower than those in standard culturing conditions."
Research Articles
- Comparison of hypoxia- and hyperoxia-induced alteration of epigene expression pattern in lungs of Pleurodeles waltl and Mus musculus
Hasan et al. 2024
Research Article
10.1371/journal.pone.0299661
- "Here, we compared the differential epigene expression pattern between Pleurodeles waltl and Mus musculus (commonly known as Iberian ribbed newt and mouse, respectively) exposed to hypoxia and hyperoxia."
- "The exposure to hyperoxia did not significantly change the expression of any gene in either newts or mice."
-
Review Articles
- Cellular adaptation to hypoxia through HIFs and beyond
Lee et al. 2020
Author Manuscript
- "Importantly, changes in O2 can also be sensed via other stress pathways as well as changes in metabolite levels and the generation of reactive oxygen species (ROS) by mitochondria."
- "Collectively, this leads to cellular adaptations of protein synthesis, energy metabolism, mitochondrial respiration, lipid and carbon metabolism as well as nutrient acquisition. "
- Technical Feasibility and Physiological Relevance of Hypoxic Cell Culture Models
Pavlacký et al. 2020
Review Article
- "Animal models in vivo fail to recapitulate some of the key hallmarks of human physiology, which leads to human cell cultures; however, they are prone to bias, namely when pericellular oxygen concentration (partial pressure) does not respect oxygen dynamics in vivo."
- "Therefore, in this review, we present evidence mandating for the close control of oxygen levels in cell culture models of hypoxia."