Equipment and Materials

Chapter 10

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Chapter 10
Last Updated
2026-06-01
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3 minute(s)
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Equipment and Materials

A.

FDA does not specifically endorse any of commercial products listed. Equivalent products may be available.

  1. Sterile swab: The following or equivalent: a. 3M™ Swab-sampler in 10 ml D/E neutralizing broth (catalog# RS96010DE, www.mmm.com) (Polyester). b. Puritan® dry cotton swab (catalog# 25-806 1PC, 25-806 2PC, www.puritanmedproducts.com) (Cotton). c. World Bioproducts PUR-Blue™ swab sampler with (catalog# BLU-10DE) or without (catalog# BLU-DRY, www.worldbioproducts.com) 10 ml D/E neutralizing broth (Polyurethane). d. Healthlink® dry swab transporter (catalog# 4159BX, www.hardydiagnostics.com) (Polyester).
  2. Sterile sponge: The following or equivalent: a. World Bioproducts EZ Reach™ sponge sampler with (catalog# EZ-10DE-PUR) or without (catalog# EZ-DRY-PUR, www.worldbioproducts.com) 10 ml D/E neutralizing broth (Polyurethane). b. Nasco Whirl-Pak® dry sponge probe (catalog# B01475WA, www.enasco.com) (Cellulose). c. 3M™ Sponge-sticks with (catalog# SSL10DE, www.mmm.com) or without (catalog# SSL100) 10 ml D/E neutralizing broth (Cellulose).

If available, dry samplers can be obtained and D/E neutralizing broth added later, or pre moistened samplers can be obtained. We do not recommend less than 10 ml D/E neutralizing broth due to the possible presence of sanitizer residues on environmental surfaces.

  1. Balance for weighing sample to 0.1 g
  2. Incubators, 30, 35, and 37°C
  3. Water bath, 80 ± 2°C
  4. Phase-contrast microscope with oil immersion phase objective (100×)
  5. Blender motor and jars or stomacher and bags
  6. Vortex mixer

Media and Reagents

B.

FDA does not specifically endorse any of commercial products listed. Equivalent products may be available.

  1. Buffered Listeria enrichment broth (BLEB) (M52)
  2. Acriflavine monohydrochloride
  3. Nalidixic acid (sodium salt)
  4. Cycloheximide
  5. Natamycin (Pimaricin)
  6. Dey/Engley (D/E) neutralizing broth (M193)
  7. Oxford medium (OXA) (M118)
  8. Polymyxin acriflavine lithium chloride ceftazidime aesculin mannitol (PALCAM) (M118a)
  9. Modified Oxford MOX agar (M103a)
  10. Lithium chloride-phenylethanol-moxalactam (LPM) agar (M81) with added esculin and iron (M82)
  11. R&F® Listeria monocytogenes Chromogenic Plating Medium (R&F Laboratories, Downers Grove, IL) (M17a)
  12. Agar Listeria according to Ottaviani and Agosti (M10a)
  13. Oxoid™ chromogenic Listeria agar (Oxoid Ltd., Basingstoke, England) (M40b)
  14. Rapid’L.mono™ (Bio-Rad Laboratories Inc.) (M131a)
  15. CHROMagar™ Listeria (CHROMagar, Paris, France) (M40a)
  16. Trypticase soy agar with 0.6% yeast extract (TSAye) (M153)
  17. Sheep blood agar (M135)
  18. Hydrogen peroxide solution, 3% for catalase test (R12)
  19. Gram stain kit
  20. Motility test medium (MTM, Difco™) (M103)
  21. Trypticase soy broth with 0.6% yeast extract (TSBye) (M157)
  22. Purple carbohydrate fermentation broth base (M130), containing 0.5% solutions of dextrose, esculin, maltose, rhamnose, mannitol, and xylose
  23. Physiological saline solution, 0.85% (R63)
  24. Fluorescent antibody (FA) buffer (Difco™)
  25. Listeria-typing sera Type 1 (Difco™ catalog# 223031) and Type 4 (Difco™ catalog# 223041)
  26. Listeria Antisera Set (Denka Seiken™ catalog# 294616)
  27. Optional: Nitrate reduction medium (M108) and nitrate detection reagents (R48)

Alternative companies may be used when the products are equivalent.