Lab Notebook Structure
Terminology
- Gametophyte
- Haploid (n) stage.
- Sporophytes
- Diploid (2n) stage.
- Phloem
"Flow-em"
- Alive, transports sugars.
- Xylem
- Dead, transports water/minerals.
- Pneumatophores
Air Roots
- Roots that extend above the soil for gas exchange.
Final Exam Questions
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What is a community in a biological context?
- All organisms of different species living close enough for potential interaction
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Why do owls produce pellets?
- They cannot pass whole bones and claws safely through their digestive tract
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What does owl pellet analysis reveal about owls?
- What the owl has fed on and prey diversity
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According to the principle discussed in this lab, what happens to diversity as you move toward the equator?
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What is the relationship between diversity and stability in an ecosystem?
- Increased diversity = increased stability
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Barn owls swallow small birds and rodents whole, and their pellets generally contain complete skeletons.
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Owls have teeth for grinding bones like predatory mammals such as wolves.
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Owl pellets are not useful to scientists.
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If a predator depends on only one prey type, a decline in that prey will not affect the predator significantly.
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Alaskan owls feed almost exclusively on lemmings, making them vulnerable to lemming population crashes.
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What are organisms called that can remove CO₂ from the atmosphere and bind it to organic molecules?
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Which process do plants use to convert CO₂ to glucose using light energy?
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What color is bromothymol blue at acidic pH (indicating high CO₂)?
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Which metabolic process do heterotrophs use to break down organic carbon and produce CO₂?
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What happens when CO₂ dissolves in water?
- It forms carbonic acid and lowers pH
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Photoautotrophs are the primary producers of organic carbon in most ecosystems.
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Decomposers such as fungi and bacteria play an important role in converting organic carbon back to CO₂.
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Plants only perform photosynthesis and never release CO₂.
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Atmospheric CO₂ levels have increased more than 40% since preindustrial times (1700s).
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Greenhouse gases like CO₂ trap heat by blocking infrared radiation from escaping to space.
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Vertebrates belong to which phylum?
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Which characteristic distinguishes vertebrates from other chordates?
- Nerve fibers enclosed in a protective column of bone (spinal cord)
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What type of body cavity do vertebrates have?
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How many chambers does the mammalian heart have?
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Which term describes the direction toward the back of the body?
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All vertebrates, including pigs, are triploblastic deuterostomes with bilateral symmetry
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Mammals are characterized by mammary glands, the ability to give birth to live young, and endothermic temperature regulation.
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Vertebrates have a closed circulatory system with a centralized pump (heart) that improves circulation.
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The diaphragm is part of the digestive system.
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Mammals and birds have a four-chambered heart and separate circulatory circuits for the lungs versus the rest of the body.
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What distinguishes animals from other multicellular organisms?
- They are heterotrophs without cell walls
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What are the specialized cells in sponges that engulf food particles from water?
- Collar cells (choanocytes)
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Which phylum includes jellies, corals, and sea anemones?
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What type of body cavity do nematodes (roundworms) have?
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What structure in mollusks contains backward-curved teeth used for scraping up food?
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Sponges are sessile filter feeders that draw water through pores into a central cavity called the spongocoel.
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Flatworms (Platyhelminthes) are acoelomate and lack both circulatory and respiratory systems.
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Cnidarians have specialized stinging cells called cnidocytes that contain nematocysts.
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Arthropods have a hard exoskeleton made of chitin and paired, jointed appendages.
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Crustaceans are the only group of arthropods with two pairs of antennae.
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What is the primary mode of nutrition in fungi?
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What are the tubular filaments that make up the body of most multicellular fungi called?
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Which fungal phylum includes the common black bread mold Rhizopus?
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What structure in Ascomycetes is the sac-like structure where sexual spores (ascospores) are produced?
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What is the fruiting body of a Basidiomycete (like a mushroom) called?
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Fungal cell walls are usually made of chitin, the same polysaccharide found in insect exoskeletons.
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Hyphae that lack septa (cross-walls) are termed coenocytic and are multinucleate.
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Lichens are mutualistic associations between a fungus and a photosynthetic alga or cyanobacterium.
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Fungi store food in the form of starch, similar to plants.
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Mycorrhizae are symbiotic associations between fungi and plant roots that help plants extract minerals from soil.
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What are organisms called that can manufacture their own food using photosynthesis?
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What are the two main types of chlorophyll found in plants?
- Chlorophyll a and chlorophyll b
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What is the Rf value in paper chromatography?
- The ratio of the distance a substance moves to the distance the solvent front travels
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In paper chromatography with a nonpolar solvent, which type of substance moves fastest?
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What is the function of accessory pigments like carotenoids?
- They absorb different wavelengths than chlorophyll and pass energy to chlorophyll
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Photosynthesis occurs in membrane-bound structures called chloroplasts.
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Molecules that absorb energy in the visible portion of the electromagnetic spectrum are called pigments.
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In paper chromatography, polar substances are more attracted to the polar chromatography paper and move slower.
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The solvent used in the lab (90% petroleum ether) is a polar solvent.
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Photosynthesis is divided into light-dependent reactions and light-independent reactions (Calvin cycle).
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What are the four layers of a tropical rainforest from top to bottom?
- Emergent, canopy, understory, forest floor
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Which group of plants are seedless vascular plants that reproduce by means of spores?
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What are epiphytes?
- Plants that grow on other plants without harming them
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What are pneumatophores?
- Modified roots that extend above water/soil for gas exchange
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In cycads, what feature indicates that water is required for fertilization?
- Their sperm are flagellated
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The Missouri Botanical Garden is the oldest botanical garden in the United States, founded in 1859.
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Tropical rain forests form a belt around the equatorial regions and are the most diverse terrestrial regions on Earth.
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Cycads are angiosperms that are closely related to palms.
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Cactus spines are modified leaves that help reduce water loss.
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Fern spores are formed in structures called sporangia, usually found clustered on the underside of leaves.
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What does the term 'angiosperm' literally mean?
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Which venation pattern is characteristic of eudicots?
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Which type of root system is primarily found in eudicots?
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What structures control gas exchange in leaves by opening and closing?
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What term describes a flower that has both male (stamens) and female (carpels) reproductive parts?
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Monocots typically have parallel leaf venation while eudicots have net venation.
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Xylem cells are alive at functional maturity and transport sugars throughout the plant
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The cuticle is a waxy coating on leaf epidermis that helps prevent excess water loss.
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An aggregate fruit (like a strawberry) develops from the ovary of a single flower.
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Parenchyma cells are the primary site of photosynthesis in leaves and can store starch.
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What characteristic distinguishes algae from land plants?
- Algae lack true vascular tissues
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Which algal group is commonly known as 'seaweeds' and can grow up to 100 meters in length?
- Phaeophytes (brown algae)
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In bryophytes (mosses), which generation is dominant?
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What structure in ferns contains clusters of sporangia on the underside of fronds?
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What does the term 'gymnosperm' literally mean?
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All algae have chlorophyll a plus either chlorophyll b, c, or d.
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In ferns, the sporophyte generation is dominant, unlike in mosses.
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Red algae (Rhodophytes) have flagellated stages in their life cycles.
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Pine trees can be distinguished from other conifers because their needles occur in bundles on dwarf branches.
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Both mosses and ferns require water for fertilization because they have flagellated sperm.
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Which domain do microbial eukaryotes belong to?
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What structure distinguishes eukaryotic cells from prokaryotic cells?
- Nuclear envelope enclosing DNA
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The traditional division of kingdom Protista into Protozoa and Algae was based on:
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Which group of protozoa moves using pseudopodia?
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What term describes organisms that engulf their food by phagocytosis?
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Kingdom Protista is a monophyletic group (includes an ancestral species and all its descendants).
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Ciliates have cells with two nuclei and contractile vacuoles.
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Mixotrophic organisms can switch between autotrophic and heterotrophic metabolism.
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All protozoa are free-living organisms and none are parasitic.
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Absorptive heterotrophs digest their food extracellularly by secreting digestive enzymes.
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The pGLO plasmid contains a gene for beta-lactamase. What is the function of this enzyme?
- It cleaves ampicillin, providing antibiotic resistance
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From which organism was the GFP gene originally obtained?
- Aequorea victoria jellyfish
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Why are bacteria treated with calcium chloride and heat-shocked during transformation?
- To enhance plasmid uptake into cells
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A gene promoter initiates transcription. Which promoter type is attached to the beta-lactamase gene on the pGLO plasmid?
- A promoter that is always on
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Why don't bacteria on the LB/amp +DNA plate glow under UV light?
- The arabinose inducer is not present
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In this experiment, arabinose acts as an inducer that turns on the GFP gene.
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All bacteria in the +DNA tube will successfully take up the pGLO plasmid.
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E. coli naturally takes up free plasmid DNA from its environment without any treatment.
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The pGLO DNA solution will glow green under UV light before being transformed into bacteria.
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Bacteria that take up the pGLO plasmid can grow on plates containing ampicillin.