Transformation

CRISPR

Description
Genetic transformation using CRISPR gene editing.
Last Updated
2026-06-01
Reading Time
2 minute(s)
Word Count
208 words

Table of Contents

Ancestors

Siblings

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Techniques

Floral Dip Technique
"However, transgenes derived from the AMGT vector are randomly integrated into the host genome; this might result in the disruption of host gene expression. Such limitations of AMGT call for alternative physical or chemical methods to expand the scope of plant engineering."

Research Articles

CRISPR/Cas9 mutagenesis of the Arabidopsis GROWTH-REGULATING FACTOR (GRF) gene family Angulo et al. 2023 Research Article
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Direct protein delivery into intact Arabidopsis cells for genome engineering Furuhata et al. 2024 Research Article
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Methodology

Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana Clough et al. 1998 Methodology
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Protocol: Streamlined sub-protocols for floral-dip transformation and selection of transformants in Arabidopsis thaliana Davis et al. 2009 Methodology
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Review Articles

Good for background information and broad concepts.

Review Articles

Arabidopsis thaliana Floral Dip Transformation Method Bent et al. 2006 Review Article
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CRISPR-based gene editing in plants: Focus on reagents and their delivery tools Ebrahimi et al. 2024 Review Article
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