Techniques
- Crispr_C
- Circular vectors
- DNA plasmids
- Floral Dip Technique
- However, transgenes derived from the AMGT vector are randomly integrated into the host genome3,4; this might result in the disruption of host gene expression. Such limitations of AMGT call for alternative physical or chemical methods to expand the scope of plant engineering.
Research Articles
- CRISPR/Cas9-Based Lateral Flow and Fluorescence Diagnostics Osborn et al. 2021 Research Article
- ?
- CRISPR/Cas9 mutagenesis of the Arabidopsis GROWTH-REGULATING FACTOR (GRF) gene family Angulo et al. 2023 Research Article
- ?
- CRISPR/Cas9 targeted mutagenesis of SlLBD40, a lateral organ boundaries domain transcription factor, enhances drought tolerance in tomato Liu et al. 2020 Research Article
- ?
- Direct protein delivery into intact Arabidopsis cells for genome engineering Furuhata et al. 2024 Research Article
- ?
Review Articles
Good for background information and broad concepts.
Review Articles
- CRISPR-based gene editing in plants: Focus on reagents and their delivery tools Ebrahimi et al. 2024 Review Article
- ?
- Non-Invasive Delivery of CRISPR/Cas9 Ribonucleoproteins (Cas9 RNPs) into Cells via Nanoparticles for Membrane Transport Tashima 2025 Review Article
- ?