Techniques

Floral Dip Technique
However, transgenes derived from the AMGT vector are randomly integrated into the host genome3,4; this might result in the disruption of host gene expression. Such limitations of AMGT call for alternative physical or chemical methods to expand the scope of plant engineering.

Research Articles

CRISPR/Cas9-Based Lateral Flow and Fluorescence Diagnostics Osborn et al. 2021 Research Article
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CRISPR/Cas9 mutagenesis of the Arabidopsis GROWTH-REGULATING FACTOR (GRF) gene family Angulo et al. 2023 Research Article
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CRISPR/Cas9 targeted mutagenesis of SlLBD40, a lateral organ boundaries domain transcription factor, enhances drought tolerance in tomato Liu et al. 2020 Research Article
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Direct protein delivery into intact Arabidopsis cells for genome engineering Furuhata et al. 2024 Research Article
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Review Articles

Good for background information and broad concepts.

Review Articles

CRISPR-based gene editing in plants: Focus on reagents and their delivery tools Ebrahimi et al. 2024 Review Article
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Non-Invasive Delivery of CRISPR/Cas9 Ribonucleoproteins (Cas9 RNPs) into Cells via Nanoparticles for Membrane Transport Tashima 2025 Review Article
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