Equipment and Materials
A.
- Same as for Salmonella, Chapter 5
- Forced air incubators, maintained at 42.0 ± 0.3°C and 44.0 ± 0.3°C
- Anaerobic jar
- Anaerobic gas generating pouch/sachet
- Anaerobic indicator
B.
- Shigella broth with novobiocin (M136)
- Trypticase soy-yeast extract (TSYE) broth (M157)
- MacConkey agar (M91)
- Triple sugar iron (TSI) agar (M149)
- Urea broth (M171)
- Motility test medium (semisolid) (M103)
- Potassium cyanide (KCN) broth (M126)
- Malonate broth (M92)
- Tryptone (tryptophane) broth, 1% (M164)
- MR-VP broth (M104)
- Christensen citrate agar (M39)
- Veal infusion agar (M173)
- Bromcresol purple broth (M26) supplemented with the following carbohydrates, each at a level of 0.5%: adonitol, salicin, rhamnose, glucose, inositol, lactose, mannitol, raffinose, sucrose, xylose, dulcitol, and glycerol.
- Acetate agar (M3)
- Mucate broth (M105)
- Mucate control broth (M106)
- Decarboxylase basal medium (lysine, Falkow) (M44)
- Decarboxylase basal medium (ornithine)(M44)
Reagents and Stains
C.
- Kovacs' reagent (R38)
- Voges-Proskauer test reagents (R89)
- 1 N Sodium hydroxide solution (R73)
- 1 N Hydrochloric acid (R36)
- Methyl red indicator (R44)
- Physiological saline solution, 0.85% (sterile) (R63)
- Novobiocin
- Polyvalent Shigella antisera for groups A, A1, B, C, C1, C2, D and Alkalescens-Dispar
biotypes 1-4
- Gram stain reagents(R32)