Sterile, homogenizer blender filter bags (710 ml) or equivalent, sterile 500 ml wide
mouth, screw-cap jars, sterile 500 ml Erlenmeyer flasks, sterile 250 ml beakers, sterile
glass or paper funnels of appropriate size, and, optionally, containers of appropriate
capacity to accommodate large and composited samples
Sterile, bent glass or plastic spreader rods
Balance, 2000 g capacity, sensitivity of 0.1 g
Balance, 120 g capacity, sensitivity of 5 mg
Incubator, 35 ± 2°C
Refrigerated incubator or laboratory refrigerator, 4 ± 2°C
Water bath, 49 ± 1°C
Water bath, circulating, thermostatically-controlled, 42 ± 0.2°C and 43 ± 0.2°C
Sterile spoons or other appropriate instruments for transferring food samples
Sterile culture dishes, 15 × 100 mm, glass or plastic
Sterile pipets, 1 ml, with 0.01 ml graduations; 5 and 10 ml, with 0.1 ml graduations
Sterile inoculating needle and inoculating loop (10 µl and 1 µl)
Sterile test or culture tubes, 16 × 150 mm and 20 × 150 mm; serological tubes, 10 × 75
mm or 13 × 100 mm
Test or culture tube racks
Vortex mixer
Sterile shears, large scissors, scalpel, and forceps
Fisher or Bunsen burner
pH test paper (pH range 6-8) with maximum graduations of 0.4 pH units per color
change 22. pH meter
Plastic bags, 28 × 37 cm, sterile, with resealable tape (Items 21-22 are needed in the
analysis of frog legs and rabbit carcasses.)
Plastic beakers, 4 liter, autoclavable, for holding plastic bag during shaking and
incubation
Sponges, non-bactericidal, or equivalent
Swabs, non-bactericidal, cotton-tipped
Ultraviolet (UV) light source with an emission wavelength between 360 to 400 nm when
Green Fluorescent Protein (GFP)-UV control strains are used.
B. Media, Reagents, and Quality Control Cultures
Lactose broth (M74)
Nonfat dry milk (reconstituted) (M111)
Selenite cystine (SC) broth (M134)
Tetrathionate (TT) broth (M145)
Rappaport-Vassiliadis (RV) medium (M132). NOTE: RV medium must be made rom its
individual ingredients. Commercial formulations are not acceptable.
Salmonella somatic group (O) antisera: A, B, C1, C2, C3, D1, D2, E1, E2, E3, E4, F, G, H, I,
Vi, and other groups, as appropriate
Salmonella Spicer-Edwards flagellar (H) antisera
Modified buffered peptone water, two formulas (M192b, M192c)
Suggested Quality Control Cultures
Analysts must use both typical and atypical Salmonella controls for sample analysis,
since it may be more difficult to confirm the presence of an atypical strain in a sample
without an atypical control strain for guidance.
Green fluorescent protein (GFP) control strains which fluoresce under UVA light, more
specifically at wavelengths between 360 to 400 nm, have been developed by FDA and
have been licensed by FDA to Microbiologics for distribution.
(200 Cooper Avenue North St. Cloud, MN 56303, 1800-599-2847).
The following cultures may be purchased from Microbiologics:
Non-GFP fluorescent Salmonella control strains may also be used for sample
analysis. In addition to the positive control cultures (typical Salmonella; lactose
negative, H2S positive), 3 additional Salmonella cultures are recommended to
assist in the selection of atypical Salmonella colony morphology on selective
agars. These cultures are a lactose positive, H2S-positive S. diarizonae
(47:i:z53:z57, ATCC 12325) and a lactose-negative, H2S-negative S. Abortusequi
(4,12:-:e,n,x, ATCC 9842); or a lactose-positive, H2S-negative S. diarizonae
(60:r:e,n,x,z15, ATCC 29934). These cultures may be obtained from the American
Type Culture Collection (http://www.atcc.org/), 10801 University Boulevard,
Manassas, VA 20110-2209.