Equipment and Materials
A.
- Same basic equipment as for conventional plate count (Chapter 3).
- Drying cabinet or incubator for drying surface of agar plates 3.
Sterile bent glass streaking rods, hockey stick or hoe-shaped, with fire-polished ends, 3-4 mm diameter, 15-20 cm long, with an angled spreading surface 45-55 mm long
B.
- Baird-Parker medium (M17)
- Trypticase (tryptic) soy agar (TSA) (M152)
- Brain heart infusion (BHI) broth (M24)
- Coagulase plasma (rabbit) with EDTA
- Toluidine blue-DNA agar (M148)
- Lysostaphin (Schwartz-Mann, Mountain View Ave., Orangeburg, NY 10962)
- Tryptone yeast extract agar (M165)
- Paraffin oil, sterile
- 0.02 M phosphate-saline buffer (R61), containing 1% NaCl
- Catalase test (R12)