Do not autoclave tightly sealed containers!

Autoclave Sterilization

Make sure there are no pockets for water to get trapped.

Autoclave Sterilization

Mason Jars, Rings, and Lids

Wrap tinfoil tightly around the lid of an open jar.

?

Net Cups Cannot autoclave HDPE

Wash with soap and water.

Vermiculite

Can autoclave in a vented mason jar.

Clay Pebbles

? Can autoclave.

Use Milli-Q water, it has less bacteria in it and will help with root rot.

Autoclave Procedures

Vermiculite

Place the desired amount of dry vermiculite into the autoclavable container. Do not fill the container more than two-thirds full to allow for steam circulation.

If pre-moistened vermiculite is required, add a small amount of deionized water and mix until the vermiculite is damp but not saturated.

Cover the opening of the container with a vented closure or a double layer of aluminum foil.

Place a strip of autoclave indicator tape on the exterior of the container.

Place the container in the autoclave. Ensure there is adequate space between multiple containers.

Run the autoclave on a standard gravity or liquid cycle at 121°C and 15 psi for at least 60 minutes.[15][16] For larger volumes, a longer duration of 90 minutes is recommended.

After the cycle is complete, allow the autoclave to cool and depressurize completely before opening.

Carefully remove the sterilized vermiculite and let it cool to room temperature in a sterile environment (e.g., a laminar flow hood) before use.