Do not autoclave tightly sealed containers!

Autoclave Sterilization

Make sure there are no pockets for water to get trapped.

Autoclave Sterilization

Mason Jars, Rings, and Lids
Wrap tinfoil tightly around the lid of an open jar.
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Net Cups Cannot autoclave HDPE
Wash with soap and water.
Vermiculite
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Clay Pebbles
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Use Milli-Q water, it has less bacteria in it and will help with root rot.