Commentaries

Oxygen and mammalian cell culture: are we repeating the experiment of Dr. Ox? Ast et al. 2019 Commentary

"Oxygen tensions vary widely across tissues but are significantly lower than those in standard culturing conditions."

Research Articles

Comparison of hypoxia- and hyperoxia-induced alteration of epigene expression pattern in lungs of Pleurodeles waltl and Mus musculus Hasan et al. 2024 Research Article 10.1371/journal.pone.0299661

"Here, we compared the differential epigene expression pattern between Pleurodeles waltl and Mus musculus (commonly known as Iberian ribbed newt and mouse, respectively) exposed to hypoxia and hyperoxia."

"The exposure to hyperoxia did not significantly change the expression of any gene in either newts or mice."

Review Articles

Cellular adaptation to hypoxia through HIFs and beyond Lee et al. 2020 Author Manuscript

"Importantly, changes in O2 can also be sensed via other stress pathways as well as changes in metabolite levels and the generation of reactive oxygen species (ROS) by mitochondria."

"Collectively, this leads to cellular adaptations of protein synthesis, energy metabolism, mitochondrial respiration, lipid and carbon metabolism as well as nutrient acquisition. "

Technical Feasibility and Physiological Relevance of Hypoxic Cell Culture Models Pavlacký et al. 2020 Review Article

"Animal models in vivo fail to recapitulate some of the key hallmarks of human physiology, which leads to human cell cultures; however, they are prone to bias, namely when pericellular oxygen concentration (partial pressure) does not respect oxygen dynamics in vivo."

"Therefore, in this review, we present evidence mandating for the close control of oxygen levels in cell culture models of hypoxia."